Department of  Neurosurgery
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Itender Singh, PhD

Assistant Professor of Neurological Surgery

Washington University School of Medicine
Department of Neurological Surgery

660 S. Euclid Avenue
Campus Box 8057
St. Louis, Missouri 63110

Phone: 314-362-4476

Singh Laboratory: Translational Neuroscience

Research Interests
For the past decade, the central theme of Dr. Itender Singh’s research has been studying the molecular basis of neurodegeneration and translating his observations into therapeutic strategies. Currently he is investigating the neurovascular mechanisms of neurodegeneration in Alzheimer’s disease (AD), ischemic stroke, subarachnoid hemorrhage (SAH), germinal matrix hemorrhage (GMH) and flavivirus infections. He is also engaged in identifying and developing translational biomarkers and therapeutic targets for AD, SAH and GMH. 

B.S., Biology, M.D. University, Rohtak, India, 1995
M.S., Zoology (Parasitology), C.C.S. University, Meerut, India, 1997
M.Phil., Biotechnology, University of Delhi, New Delhi, India, 1999
Ph.D., Medical Microbiology, University of Delhi, New Delhi, India, 2004

Academic Positions
Junior Research Fellow, Department of Microbiology, University of Delhi, New Delhi, India, 1999-2000

Senior Research Fellow, Department of Microbiology, University of Delhi, New Delhi, India, 2000-2002

Visiting Research Specialist, Center for Lung and Vascular Biology, Department of Pharmacology, Chicago, IL, 2003-2006

Postdoctoral Research Associate, Center for Neurodegenerative and Vascular Brain Disorders, Department of Neurological Surgery, Rochester, NY, 2006-2012

Research Assistant Professor, Department of Pediatrics, University of Rochester Medical Center, Rochester, NY, 2012-2014

Assistant Professor, Department of Neurological Surgery, Washington University School of Medicine, St. Louis, MO, 2014-present

Selected Peer-Reviewed Publications

  1. Reynolds MR, Singh I*, Azad TD, Holmes BB, Verghese PB, Dietrich HH, Diamond M, Bu G, Han BH, Zipfel GJ. Heparan sulfate proteoglycans mediate Aβ-induced oxidative stress and hypercontractility in cultured vascular smooth muscle cells. Molecular Neurodegeneration, 2016, 11:9. *Co-first author
  2. Han BH, Zhou M, Johnson AW, Singh I, Liao F, Vellimana AK, Nelson JW, Milner E, Cirrito JR, Basak J, Yoo M, Dietrich HH, Holtzman DM, Zipfel GZ. Contribution of reactive oxygen species to cerebral amyloid angiopathy, vasomotor dysfunction, and microhemorrhage in aged Tg2576 mice. Proceedings of the National Academy of Sciences USA (PNAS), 2015, 112(8):E881-90.
  3. Iliff JJ, Chen MJ, Plog BA, Zeppenfeld DM, Soltero M, Yang L, Singh I, Deane R, Nedergaard M. Impairment of glymphatic pathway function promotes tau pathology after traumatic brain injury. Journal of Neuroscience, 2014,  3;34(49):16180-93.
  4. Singh I, Sagare AP, Coma M, Perlmutter D, Gelein R, Bell RD, Deane RJ, Zhong E, Parisi M, Ciszewski J, Kasper RT, Deane R. Low levels of copper disrupt brain amyloid-β homeostasis by altering its production and clearance. Proceedings of the National Academy of Sciences USA (PNAS), 2013, 110(36):14771-14776.
  5. Deane R, Singh I*, Sagare AP, Bell RD, Ross NT, Larue B, Love R, Perry S, Paquette N, Deane RJ, Thiyagarajan M, Zarcone T, Fritz G, Friedman AE, Miller BL, Zlokovic BV. A multimodal RAGE-specific inhibitor reduces amyloid β-mediated brain disorder in a mouse model of Alzheimer disease. Journal of Clinical Investigation, 2012, 122(4): 1377-1392. *Co-first author
  6. Bell RD, Winkler EA, Singh I, Sagare AP, Deane R, Wu Z, Holtzman DM, Betsholtz C, Armulik A, Sallstrom J, Berk BC, Zlokovic BV. Apolipoprotein E controls cerebrovascular integrity via cyclophilin A. Nature, 2012, 485(7399): 512-516.
  7. Zhu D, Wang Y, Singh I*, Bell RD, Deane R, Zhong Z, Sagare A, Winkler EA, Zlokovic BV. Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor. Blood, 2010, 115(23): 4963-4972. PMC ID: PMC2890172. *Co-first author
  8. Bell RD, Winkler EA, Sagare AP, Singh I, LaRue B, Deane R, Zlokovic BV. Pericyte control key neurovascular functions and neuronal phenotype in the adult brain and during brain ageing. Neuron, 2010, 68(3): 409-427.
  9. Zhong Z, Ilieva H, Hallagan L, Bell R, Singh I, Paquette N, Thiyagarajan M, Deane R, Fernandez JA, Lane S, Zlokovic AB, Liu T, Griffin JH, Chow N, Castellino F, Stojanovic K, Cleveland DW, Zlokovic BV. Activated protein C therapy slows ALS-like disease in mice by transcriptionally inhibiting SOD1 in motor neurons and microglia cells. Journal of Clinical Investigation, 2009, 119(11): 3437-3449.
  10. Fernández JA, Heeb MJ, Xu X, Singh I, Zlokovic BV, Griffin JH. Species-specific anticoagulant and mitogenic activities of murine protein S. Haematologica, 2009, 94(12): 1721-1731.
  11. Bell RD, Deane R, Chow N, Long X, Sagare A, Singh I, Streb JW, Guo H, Rubio A, Van Nostrand W, Miano JM, Zlokovic BV. SRF and myocardin regulate LRP-mediated amyloid-beta clearance in brain vascular cells. Nature Cell Biology, 2009, (2): 143-153.
  12. Wang Y, Thiyagarajan M, Chow N, Singh I, Guo H, Davis TP, Zlokovic BV. Differential neuroprotection and risk for bleeding from activated protein C with varying degrees of anticoagulant activity. Stroke, 2009, 40(5): 1864-1869.
  13. Guo H, Singh I*, Wang Y, Deane R, Barrett T, Fernández JA, Chow N, Griffin JH, Zlokovic BV. Neuroprotective activities of activated protein C mutant with reduced anticoagulant activity. European Journal of Neuroscience, 2009, 29(6): 1119-1130. *Co-first author
  14. Guo H, Wang Y, Singh I, Liu D, Fernández JA, Griffin JH, Chow N, Zlokovic BV. Species-dependent neuroprotection by activated protein C mutants with reduced anticoagulant activity. Journal of Neurochemistry, 2009, 109(1): 116-124.
  15. Singh I, Knezevic N, Ahmmed GU, Kini V, Malik AB, Mehta D.Galphaq-TRPC6-mediated Ca2+ entry induces RhoA activation and resultant endothelial cell shape change in response to thrombin. Journal of Biological Chemistry, 2007, 282(11): 7833-7843.
  16. Singh I and Virdi JS. Interaction of Yersinia enterocolitica biotype 1A strains of diverse origin with cultured cells in vitro. Japanese Journal of Infectious Disease, 2005, 58: 31-33.
  17. Singh I and Virdi JS. Production of Yersinia stable toxin (YST) and distribution of yst genes in biotype 1A strains of Yersinia enterocolitica. Journal of Medical Microbiology, 2004, 53: 1065-1068.
  18. Singh I and Virdi JS. In vitro antibiotic susceptibilities of Yersinia enterocolitica biotype 1A. World Journal of Microbiology & Biotechnology, 2004, 20: 329-331.