Washington University School of Medicine Logowustl.edu

Henry Han, PhD

Assistant Professor of Neurological Surgery

Washington University School of Medicine
Department of Neurological Surgery
660 S. Euclid Avenue
Campus Box 8057
St. Louis, Missouri 63110

Phone: 314-362-4476
Fax: 314-362-2107
E-mail: hanh@wudosis.wustl.edu

Research Interests
Cellular and molecular mechanisms underlying neurovascular degeneration and dysfunction associated with cerebrovascular disorders including cerebral amyloid angiopathy, aneurysmal subarachnoid hemorrhage and ischemic stroke. Exploring the neurovascular protective effects of phosphodiesterases, anti-inflammatory flavonoids, hypoxic preconditioning and growth factors in rodent models of cerebrovascular disease. Development of PET imaging tracers for the diagnosis of cerebral amyloid angiopathy.

Education
BS, Pharmacy
Seoul National University, Seoul, Korea, 1985

MS, Pharmacology
Seoul National University, Seoul, Korea, 1987

PhD, Pharmacology
University of Missouri, Columbia, Missouri, 1997

Academic Positions
Postdoctoral research fellow, Department of Neurology, Washington University School of Medicine (1998-2001)

Research Instructor, Department of Neurology, Washington University School of Medicine (2001-02)

Assistant Professor, Department of Pharmacology, Seoul National University, Seoul, Korea (2002-06)

Research Assistant Professor, Department of Neurological Surgery, Washington University School of Medicine (2006-2011)

Assistant Professor, Department of Neurological Surgery, Washington University School of Medicine (2011 – present)

Publications

  1. Chen B, Han BH, Sun XH, Lim RW. Inhibition of muscle-specific gene expression by Id3: requirement of the C-terminal region of the protein for stable expression and function. Nucleic Acids Research 25: 423-430, 1997.
  2. Han BH, Park DJ, Lim RW, Im JH, Kim HD. Cloning, expression, and characterization of novel guanylate-binding protein, GBP3 in murine erythroid progenitor cells. Biochim Biophysic Acta 1484: 373-386, 1998.
  3. Han BH. Interferon-gamma and lipopolysaccharide induce mouse guanylate-binding protein 3 (mGBP3) in the murine macrophage cell line. Archives of Pharmacal Research 22: 130-136, 1999.
  4. Joo NS, Clarke LL, Han BH, Forte LR, Kim HD. Cloning of CLC-2 chloride channel from murine duodenum and its presence in CFTR knockout mice. Biochimica et Biophysica Acta 1446: 431-437, 1999.
  5. Selznick LA, Holtzman DM, Han BH, Gokden M, Srinivasan AN, Johnson EM, Jr, Roth KA. In situ immunodetection of neuronal caspase-3 activation in Alzheimer’s disease. J Neuropathol Exp Neurol 58: 1020–1026, 1999.
  6. Han BH, D’Costa A, Parsadanian M, Shah AR, Gidday J, Patel S, Srinivasan A, Deshmukh M, Holtzman DM. BDNF blocks caspase-3 activation in neonatal hypoxia-ischemia. Neurobiol Dis 7: 38-53, 2000.
  7. Kim HD, Burnett J, Turner J, Joo NS, Han BH, Lim R, Bowen JW, Kirk RG, Lee P. Stimulation of K transport by furosemide in HCD57 erythroid cells. J Membr Biol 175: 235-244, 2000.
  8. Lendon CL, Han BH, Salimi K, Fagan AM, Behrens MI, Muller MC, Holtzman DM. No effect of apolipoprotein E on neuronal cell death due to excitotoxic and apoptotic agents in vitro and neonatal hypoxic ischemia in vivo. Eur J Neurosci 12: 2235-2242, 2000.
  9. Almli CR, Levy T, Han BH, Shah A, Gidday J, Holtzman DM. BDNF protects against spatial memory deficits following neonatal hypoxia-ischemia. Exp Neurol 166: 99-114, 2000.
  10. Han BH, Holtzman DM. BDNF protects the neonatal brain from hypoxic-ischemic injury in vivo via the ERK pathway. J Neurosci 20: 5775-81, 2000.
  11. Holtzman DM, Han BH, He YY, Kim KM, Choi J, Hsu C. Reply to ‘Inhibition of post-ischemic brain injury by clusterin overexpression.' Nat Med 7: 978-979, 2001.
  12. Gibson ME, Han BH, Choi J, Knudson CM, Korsmeyer SJ, Parsadanian M, Holtzman DM. BAX contributes to apoptotic-like death following neonatal hypoxia-ischemia: Evidence for distinct apoptosis pathways. Mol Med 7: 644-655, 2001.
  13. Han BH, DeMattos RB, Dugan LL, Kim-Han JS, Brenza R, Fryer JD, Kierson M, Cirrito J, Quick K, Harmony J, Aronow B, Holtzman DM. Clusterin contributes to caspase-3 independent brain injury following neonatal hypoxia-ischemia. Nat Med 7: 338-343, 2001.
  14. Back SA, Han BH, Luo NL, Chricton CA, Arvin KL, Holtzman DM. Selective vulnerability of late oligodendrocyte progenitors to hypoxia-ischemia. J Neurosci 22: 455-463, 2002.
  15. Han BH, Xu D, Choi J, Han Y, Xanthoudakis S, Roy S, Tam J, Vaillancourt J, Colucci J, Giroux A, Robertson GS, Zamboni B, Nicholson DW, Holtzman DM. Selective non-peptide caspase inhibitor is neuroprotective and reveals distinct pathways of cell death following neonatal hypoxic-ischemic brain injury. J Biol Chem 277: 30128-30136, 2002.
  16. Arvin KL, Han BH, Paul S, Du Y, Holtzman DM. Minocycline markedly protects the neonatal brain against hypoxia-ischemia. Annal Neurol 52: 54-61, 2002.
  17. Han BH, Choi J, Holtzman DM. Evidence that p38 mitogen-activated protein kinase contributes to neonatal hypoxic-ischemic brain injury. Dev Neurosci 24: 405-410, 2002.
  18. Manabat C, Han BH, Wendland M, Derugin N, Holtzman DM, Ferriero DM, Vexler ZS. Reperfusion differentially induces caspase-3 activation in ischemic core and penumbra after focal ischemia in immature brain. Stroke 34: 207-213, 2003.
  19. Kang SS, Lee JY, Choi YK, Kim GD, Han BH. Neuroprotective Effects of Flavones on Hydrogen Peroxide-induced Apoptosis in SH-SY5Y Neuroblostoma Cells. Bioorg Med Chem Lett 14: 2261-2264, 2004.
  20. Choi JW, Shin CY, Yoo BK, Choi MS, Lee WJ, Han BH, Kim WK, Kim HC, Ko KH. Glucose deprivation increases hydrogen peroxide level in immunostimulated rat primary astrocytes. J Neurosci Res. 75: 722-31, 2004.
  21. Choi WS, Eom D, Han BS, Kim WK, Han BH, Choi EJ, Oh TH, Markelonis GJ, Cho JW, Oh YJ. Phosphorylation of p38 MAPK induced by oxidative stress is linked to activation of both caspase-8- and –9-mediated apoptotic pathways in dopaminergic neurons. J Biol Chem 279: 20451-60, 2004.
  22. Ju C, Oh YJ, Han BH, Kim HS, Kim HC, Kim WK. Intracellular pH-dependent peroxynitrite-evoked synergistic death of glucose-deprived astrocytes. Free Radic Biol Med. 37: 1160-9, 2004.
  23. Hwang IK, Yoo KY, Kim DS, Do SG, Oh YS, Kang TC, Han BH, Ki JS, Won MH. Expression and changes of galanin in neurons and microglia in the hippocampus after transient forebrain ischemia in gerbils. Brain Res 1023: 193-9, 2004.
  24. Hwang IK, Yoo KY, Kim DW, Han BH, Kang TC, Choi SY, Kim JS, Won MH. Protein disulfide isomerase immunoreactivity and protein level changes in neurons and astrocytes in the gerbil hippocampal CA1 region following transient ischemia. Neurosci Lett 375: 117-22, 2005.
  25. Yoo BK, Choi JW, Han BH, Kim WK, Kim HC, Ko KH. Role of MAPK/ERK1/2 in the glucose deprivation-induced death in immunostimulated astroglia. Neurosci Lett 376: 171-6, 2005.
  26. Shin H, Hwang IK, Yoo KY, Song JH, Jung JY, Kang TC, Choi, SY, Han BH, Kim JS, Won MH. Expression and changes of Ca2+-ATPase in neurons and astrocytes in the gerbil hippocampus after transient forebrain ischemia. Brain Res 1049:43-51, 2005.
  27. Hwang IK, Lee KY, Yoo KY, Kim DS, Lee NS, Jeong YG, Kang TC, Han BH, Kim JS, Won MH. Tyrosine kinase A but not phosphacan/protein tyrosine phosphatase-zeta/beta immunoreactivity and protein level changes in neurons and astrocytes in the gerbil hippocampus proper after transient forebrain ischemia. Brain Res 1036: 35-41, 2005.
  28. Kim SW, Her SJ, Park SJ, Kim D, Park KS, Lee HK, Han BH, Kim MS, Shin CS, Kim SY. Ghrelin stimulates proliferation and differentiation and inhibits apoptosis in osteoblastic MC3T3-E1 cells. Bone 37: 359-369, 2005.
  29. Kang SS, Lee JY, Choi YK, Song SS, Kim JS, Jeon SJ, Han YN, Son KH, Han BH. Neuroprotective effects of naturally occurring biflavonoids. Bioorg Med Chem Lett 15:3588-3591, 2005.
  30. Kim GS, Choi YK, Song SS, Kim WK, Han BH. MKP-1 contributes to oxidative stress-induced apoptosis via inactivation of ERK1/2 in SH-SY5Y cells. Biochem Biophys Res Comm 338: 1732-1738, 2005.
  31. Shin DH, Bae YC, Kim-Han JS, Lee JH, Son KH, Kang SS, Kim WK, Han BH. Polyphenol amentoflavone affords neuroprotection against neonatal hypoxic-ischemic brain damage via multiple mechanisms. J Neurochem 96: 561-572, 2006.
  32. Park GH, Ryu JR, Shin CY, Choi MS, Han BH, Kim WK, Ko KH. Evidence that protease-activated receptor-2 mediates trypsin-induced reversal of stellation in cultured rat astrocytes. Neurosci Res 54: 15-23, 2006.
  33. Hwang IK, Yoo KY, Kim DW, Lee BH, Kang TC, Choi SY, Han BH, Kim JS, Won MH. Ischemia-related changes of glial-derived neurotrophic factor and phosphatidylinositol 3-kinase in the hippocampus: their possible correlation in astrocytes. Brain Res 1072:215-223, 2006.
  34. Shin DH, Bae YC, Kim-Han JS, Lee JH, Son KH, Kang SS, Kim WK, Han BH. Polyphenol amentoflavone affords neuroprotection against neonatal hypoxic-ischemic brain damage via multiple mechanisms. J Neurochem 96: 561-572, 2006.
  35. Wei L, Han BH, Li Y, Keogh C, Holtzman DM, Yu SP. Cell death mechanism and protective effect of erythropoietin after focal ischemia in the whisker-barrel cortex of neonatal rats. J Pharm Exp Ther 317;109-116, 2006.
  36. Han BH, Abousaleh F, Brendza RP, Koenigsknecht-Talboo J, Dietrich HH, Holtzman DM, Zipfel GJ. Cerebrovascular dysfunction precedes structural disruption of vascular smooth muscle cells in a mouse model of cerebral amyloid angiopathy: γ-secretase inhibition restores cerebrovascular function. J Neurosci 28:13542-13550, 2008.
  37. Zipfel GJ, Han BH, Ford AL, Lee J-M. Cerebral amyloid angiopathy: progressive disruption of the neurovascular unit. Stroke 16:S16-S19, 2008.
  38. Dietrich HH, Xiang C, Han BH, Zipfel GJ, Holtzman DM. Soluble amyloid-beta, effect on cerebral arteriolar regulation and vascular cells. Molecular Neurodegeneration 5:15, 2010.
  39. Han BH, Zhou ML, Vellimana AK, Milner E, Kim DH, Greenberg JK, Chu W, Mach RH, Zipfel GJ. Resorufin analogs preferentially bind cerebrovascular amyloid: potential use as imaging ligands for cerebral amyloid angiopathy. Mol Neurodegener 6:86, 2011.
  40. Han BH, Zhou ML, Milner E, Vellimana AK, Zipfel GJ. Phosphodiesterase 5 Inhibition Attenuates Cerebral Vasospasm and Improves Functional Recovery Following Subarachnoid Hemorrhage. Neurosurgery 70:178-187, 2012.
  41. Han BH, Zhou ML, Vellimana AK, Ling Y, Milner E, Cirrito JR, Basak J, Dietrich HH, Holtzman DM,  Zipfel GJ. Inhibition of NADPH oxidases reduces cerebrovascular dysfunction and microhemorrhage induced by cerebral amyloid angiopathy in mice. (submitted).
  42. Vellimana AK, Zhou ML, Milner E, Zipfel GJ, Han BH. Matrix Metalloproteinase–9 plays a causal role in SAH-induced neurovascular dysfunction (in preparation).